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Structure-Guided Modeling and Binding Studies of GABAA Receptor Subunit Beta-3

by B. Rajasekhara Reddy, L. Pratap Reddy, Sravani Saragandla
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International Journal of Computer Applications
Foundation of Computer Science (FCS), NY, USA
Volume 59 - Number 15
Year of Publication: 2012
Authors: B. Rajasekhara Reddy, L. Pratap Reddy, Sravani Saragandla
10.5120/9627-4275

B. Rajasekhara Reddy, L. Pratap Reddy, Sravani Saragandla . Structure-Guided Modeling and Binding Studies of GABAA Receptor Subunit Beta-3. International Journal of Computer Applications. 59, 15 ( December 2012), 43-46. DOI=10.5120/9627-4275

@article{ 10.5120/9627-4275,
author = { B. Rajasekhara Reddy, L. Pratap Reddy, Sravani Saragandla },
title = { Structure-Guided Modeling and Binding Studies of GABAA Receptor Subunit Beta-3 },
journal = { International Journal of Computer Applications },
issue_date = { December 2012 },
volume = { 59 },
number = { 15 },
month = { December },
year = { 2012 },
issn = { 0975-8887 },
pages = { 43-46 },
numpages = {9},
url = { https://ijcaonline.org/archives/volume59/number15/9627-4275/ },
doi = { 10.5120/9627-4275 },
publisher = {Foundation of Computer Science (FCS), NY, USA},
address = {New York, USA}
}
%0 Journal Article
%1 2024-02-06T21:04:19.891930+05:30
%A B. Rajasekhara Reddy
%A L. Pratap Reddy
%A Sravani Saragandla
%T Structure-Guided Modeling and Binding Studies of GABAA Receptor Subunit Beta-3
%J International Journal of Computer Applications
%@ 0975-8887
%V 59
%N 15
%P 43-46
%D 2012
%I Foundation of Computer Science (FCS), NY, USA
Abstract

The major inhibitory GABA systems are known to take part a vital role in epilepsy, associated with excessive neuronal circuitry excitation. This excitatory action reflects seizures which result from GABA inhibitory circuit dysfunction. GABA mediates its fast inhibition through GABAA receptors by activating it and then opens the chloride channels. This opening allows chloride ions to flow into the interior of the cell which inhibits the excitability. This inhibitory action devises anticonvulsive properties as a consequence. So GABAA receptors are primary targets in the pathophysiology of epilepsy. Enhancement in the the action of GABAA receptors is the basis of epileptic seizures reduction on which many antiseizure drugs act. Modulation of GABAA receptor can be shown by many synthetic and natural compounds. Valerenic acid, a plant origin compound shows modulatory effects on GABAA receptors. In our present study, the homology model of the GABAA receptor subunit beta-3 is build and docking studies are performed with Valerenic acid. Docking analysis is done to know the binding interactions and their binding affinity. The results revealed the interacting amino acids which are involved in binding of GABAA receptor subunit beta-3 with Valerenic acid are ASP35, GLN56, PHE55, TYR120, ARG111 and TYR 54.

References
  1. McKernan RM, and Whiting PJ (1996) Which GABAA-receptor subtypes really occur in the brain? Trends Neurosci 19:139–143.
  2. Choi DW, Farb DH, and Fischbach CD (1977) Chlordiazepoxide selectively augments GABA action in spinal cord cell cultures. Nature (Lond) 269:342-344.
  3. Macdonald RL, and Barker JL (1981) Benzodiazepines specifically modulate GABA-mediated postsynaptic inhibition in cultured mammalian neurones. Nature (Lond) 271 :563-564
  4. Whiting PJ, Mckernan RM, and Wafford KA. Structure and pharmacology of vertebrate GABAA receptor subtypes. In: International Review of Neurobiology (Bradley RJ. , and Harris RA, eds. ). San Diego: Academic Press, 1995. p. 95.
  5. Mody I (2005). Aspects of the homeostaic plasticity of GABAA receptor-mediated inhibition. J Physiol 562, 37–46.
  6. Baumann SW, Baur R, and Sigel E (2002). Forced subunit assembly in ?1?2?2 GABAA receptors. Insight into the absolute arrangement. J Biol Chem 277, 46020– 46025.
  7. Fritschy J. -M. , Benke D. , Mertens S. , Oertel W. H. , Bachi T. and Mohler H. (1992) Five subtypes of type A gamma-aminobutyric acid receptors identified in neurons by double and triple immunofluorescence staining with subunitspecific antibodies. Proc. Natn. Acad. Sci. U. S. A. 89, 6726–6730.
  8. Smith GB, Olsen RW (1995) Functional domains of GABAA receptors. Trends Pharmacol Sci 16:162–168.
  9. Berezhnoy D, Gravielle MC, and Farb DH (2007) Pharmacology of the GABAA receptor, in Handbook of Contemporary Neuropharmacology (Sibley D, Hanin I, Kuhar M, and Skolnick P eds) vol 1, pp 465–568, John Wiley & Sons/Wiley- Interscience, Hoboken, NJ.
  10. Korpi, E. R. , Gründer, G. , Lüddens, H. , 2002. Drug interactions at GABAA receptors. Prog. Neurobiol. 67, 113–159.
  11. Fritschy J: Epilepsy, E/I balance and GABAA receptor plasticity. Front Mol Neurosci 2008, 1:5.
  12. Sieghart W, Sperk G. Subunit composition, distribution and function of GABAA receptor subtypes. Curr Top Med Chem 2002; 2: 795–816.
  13. Fritschy JM, Bru¨nig I. Formation and plasticity of GABAergic synapses:physiological mechanisms and pathophysiological implications. Pharmaco Ther 2003; 98: 299–323.
  14. Abagyan RA, Totrov MM, Kuznetsov DA. ICM: A New Method For Protein Modeling and Design: Applications to Docking and Structure Prediction From The Distorted Native Conformation. J. Comp. Chem. 1994; 15:488–506.
  15. Laskowski RA et al. AQUA and PROCHECK-NMR : Programs for checking the quality of protein structures solved by NMR,Journal of Biomolecular NMR. 1996, 8(4): 477-86
  16. Morris AL et al. Stereochemical quality of protein structure coordinates. Proteins. 1992, 12(4): 345-64
  17. Sippl MJ. Knowledge-based potentials for proteins. Current Opinion in Structural Biology. 1995, 5(2): 229-35
  18. Zhang Y & Skolnick J. Scoring function for automated assessment of protein structure template quality. Proteins. 2004, 57: 702
Index Terms

Computer Science
Information Sciences

Keywords

GABA GABAA receptor epilepsy antiseizure Valerenic acid

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