A fermentative hydrogen-producing strain KTSMBNL-01 was isolated from the sewage sludge and identified as Enterobacter sp. on the basis of the biochemical characteristics and 16S rRNA gene analysis. The optimization of batch conditions for the production of hydrogen by Enterobacter sp. was investigated. Various parameters namely initial pH, temperature and substrate concentrations were varied for hydrogen production. Maximum hydrogen yield (0. 86 mol/mol G) and cell growth (1. 59 g/L) was obtained at pH 7. 0 and at 37 °C. The strain was capable of producing hydrogen in the presence glucose, fructose, xylose, maltose, lactose, cellulose and starch, where the best results revealed with glucose. This study showed that Enterobacter sp. can efficiently produce H2 and it is a one more model microorganism for biohydrogen production.

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Biohydrogen Enterobacter Sp. Optimization Glucose.