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Fermentative Hydrogen Production by Enterobacter sp. KTSMBNL-01 Isolated from Municipal Sewage Sludge: Optimization Studies

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IJCA Proceedings on National Conference cum Workshop on Bioinformatics and Computational Biology
© 2014 by IJCA Journal
NCWBCB - Number 2
Year of Publication: 2014
Authors:
Pugazhendhi A
Prakash P
Poornima Priyadharsani T. K
Valsala H
Thamaraiselvi K

Pugazhendhi A, Prakash P, Poornima Priyadharsani T.k, Valsala H and Thamaraiselvi K. Article: Fermentative Hydrogen Production by Enterobacter sp. KTSMBNL-01 Isolated from Municipal Sewage Sludge: Optimization Studies. IJCA Proceedings on National Conference cum Workshop on Bioinformatics and Computational Biology NCWBCB(2):25-28, May 2014. Full text available. BibTeX

@article{key:article,
	author = {Pugazhendhi A and Prakash P and Poornima Priyadharsani T.k and Valsala H and Thamaraiselvi K},
	title = {Article: Fermentative Hydrogen Production by Enterobacter sp. KTSMBNL-01 Isolated from Municipal Sewage Sludge: Optimization Studies},
	journal = {IJCA Proceedings on National Conference cum Workshop on Bioinformatics and Computational Biology},
	year = {2014},
	volume = {NCWBCB},
	number = {2},
	pages = {25-28},
	month = {May},
	note = {Full text available}
}

Abstract

A fermentative hydrogen-producing strain KTSMBNL-01 was isolated from the sewage sludge and identified as Enterobacter sp. on the basis of the biochemical characteristics and 16S rRNA gene analysis. The optimization of batch conditions for the production of hydrogen by Enterobacter sp. was investigated. Various parameters namely initial pH, temperature and substrate concentrations were varied for hydrogen production. Maximum hydrogen yield (0. 86 mol/mol G) and cell growth (1. 59 g/L) was obtained at pH 7. 0 and at 37 °C. The strain was capable of producing hydrogen in the presence glucose, fructose, xylose, maltose, lactose, cellulose and starch, where the best results revealed with glucose. This study showed that Enterobacter sp. can efficiently produce H2 and it is a one more model microorganism for biohydrogen production.

References

  • Moriartya, P. and Honnery, D. 2009. Hydrogen's role in an uncertain energy future. Int. J. Hydrogen Energy 34, 31–39.
  • Hamilton, C. , Hiligsmann, S. , Beckers, L. , et al. , 2010. Optimization of culture conditions for biological hydrogen production by Citrobacter freundii CWBI952 in batch, sequenced-batch and semicontinuous operating mode. Int. J. Hydrogen Energy 35, 1089–1098.
  • Roy, S. , Vishnuvardhan, M. , and Das, D. , 2013. Improvement of hydrogen production by newly isolated Thermoanaerobacterium thermosaccharolyticum IIT BT-ST1. Int. J. Hydrogen Energy. 1-12, http://dx. doi. org/10. 1016/j. ijhydene. 2013. 06. 128
  • Hu, C. C. , Giannis, A. , Chen, C-L. , Qi, W. , Wang, J-Y. , 2013. Comparative study of biohydrogen production by four dark fermentative bacteria. Int. J. Hydrogen Energy 38, 15686-15692.
  • Winter, C. J. 2005. Into the hydrogen energy economy-milestone. Int. J. Hydrogen Energy 30, 681-5.
  • Saraphirom, P. and Reungsang, A. 2010. Optimization of biohydrogen production from sweet sorghum syrup using statistical methods. Int. J. Hydrogen Energy 35, 13435–13444.
  • Kapdan, I. K. and Kargi, F. 2006. Review Bio-hydrogen production from waste materials. Enzym Microb Technol 38, 569–82.
  • Wang, J. and Wan, W. 2009. Factors influencing fermentative hydrogen production: A review. Int. J. Hydrogen Energy 34, 799–811.
  • Kumar, N. and Das, D. 2001. Continuous hydrogen production by immobilized Enterobacter cloacae IIT-BT 08 using lignocellulosic materials as solid matrices. Int. J. Hydrogen Energy 29, 280–287.
  • Long, C. , Cui, J. , Liu, Z. , et al. , 2010. Statistical optimization of fermentative hydrogen production from xylose by newly isolated Enterobacter sp. CN1. Int. J. Hydrogen Energy 35, 6657-6664.
  • Singh, L. , Wahid, Z. A,, Faisal Siddiqui, M. et al. , 2013. Biohydrogen production from palm oil mill effluent using immobilized Clostridium butyricum EB6 in polyethylene glycol. Int. J. Hydrogen Energy Process Biochemistry 48, 294–298.
  • Chen, W. M. , Tseng, Z. J. , Lee, K. S. , Chang, J. S. , 2005. Fermentative hydrogen production with Clostridium butyricum CGS5 isolated from anaerobic sewage sludge. Int. J. Hydrogen Energy 30, 1063–70.
  • Chae, K-J. , Choi, M-J. , Kim, K-Y. , et al. , 2010. Selective inhibition of methanogens for the improvement of biohydrogen production in microbial electrolysis cells. Int. J. Hydrogen Energy 35, 13379–13386.
  • Oh, Y-K. , Seola, E-H. , Kim, J. R, Park S, 2003. Fermentative biohydrogen production by a new chemoheterotrophic bacterium Citrobacter sp. Y19. Int. J. Hydrogen Energy 28, 1353–1359.
  • Zhang, Y. F. and Shen, J. Q. 2006. Effect of temperature and iron concentration on the growth and hydrogen production of mixed bacteria. Int. J. Hydrogen Energy 31, 441-6.
  • Van Ginkel, S. and Sung, S. W. 2001. Biohydrogen production as a function of pH and substrate concentration. Environ Sci Technol 35, 4726-30.
  • Sambrook J, Fritsch EF, Maniatis T, Molecular cloning: a laboratory manual. 3rd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 2001.
  • Liu, F. and Fang, B. 2007. Optimization of bio-hydrogen production from biodiesel wastes by Klebsiella pneumoniae. Biotechnol J 2, 374–380.
  • Niu, K. , Zhang, X. , Tan, W-S. , Zhu, M-L. , 2010. Characteristics of fermentative hydrogen production with Klebsiella pneumoniae ECU-15 isolated from anaerobic sewage sludge. Int. J. Hydrogen Energy 35, 71–80.
  • Chittibabu, G. , Nath, K. and Das, D. 2006. Feasibility studies on the fermentative hydrogen production by recombinant Escherichia coli BL-21. Process Biochem 41, 682–68.
  • Prakashama, R. S. , Brahmaiaha, P. and Sathisha, T. 2009. Fermentative biohydrogen production by mixed anaerobic consortia: Impact of glucose to xylose ratio. Int. J. Hydrogen Energy 34, 9354–9361.
  • Temudo, M. F. , Mato, T. , Kleerebezem, R. , van Loosdrecht, M. C. M. 2009. Xylose anaerobic conversion by open-mixed cultures. Appl Microbiol Biotechnol 82, 231–239.