Call for Paper - January 2023 Edition
IJCA solicits original research papers for the January 2023 Edition. Last date of manuscript submission is December 20, 2022. Read More

Computational Analysis of Interaction between Mycobacterial Antigens Rv0679c and Rv0180c with Toll like Receptors of Human and Mouse

Print
PDF
International Journal of Computer Applications
Foundation of Computer Science (FCS), NY, USA
Year of Publication: 2016
Authors:
Rupa Lavarti, Jayasree Ganugapati, Shirisha Ratcha, Krovvidi S. R. SivaSai
10.5120/ijca2016911062

Rupa Lavarti, Jayasree Ganugapati, Shirisha Ratcha and Krovvidi S R SivaSai. Computational Analysis of Interaction between Mycobacterial Antigens Rv0679c and Rv0180c with Toll like Receptors of Human and Mouse. International Journal of Computer Applications 148(3):1-6, August 2016. BibTeX

@article{10.5120/ijca2016911062,
	author = {Rupa Lavarti and Jayasree Ganugapati and Shirisha Ratcha and Krovvidi S. R. SivaSai},
	title = {Computational Analysis of Interaction between Mycobacterial Antigens Rv0679c and Rv0180c with Toll like Receptors of Human and Mouse},
	journal = {International Journal of Computer Applications},
	issue_date = {August 2016},
	volume = {148},
	number = {3},
	month = {Aug},
	year = {2016},
	issn = {0975-8887},
	pages = {1-6},
	numpages = {6},
	url = {http://www.ijcaonline.org/archives/volume148/number3/25734-2016911062},
	doi = {10.5120/ijca2016911062},
	publisher = {Foundation of Computer Science (FCS), NY, USA},
	address = {New York, USA}
}

Abstract

Toll like receptors (TLRs) play a key role in the innate immune response to infectious agents. The Mycobacterium tuberculosis bacilli and its antigens interaction with TLR-2-1 and TLR-4-MD2 were shown to activate intracellular signalling which determines the outcome of the disease. Present study focused on two new antigens Rv0679c and Rv0180c of M.tb H37Rv and their interactions with human and mouse TLRs using computational approach. Structures of Rv0679c and Rv0180c antigens of M.tb were generated using I-TASSER and docked with TLR-2-1 and TLR-4-MD2 complexes of human and mouse using Cluspro2.0. Rv0180c antigen has better binding energy to both human and mouse TLR-2-1 and TLR-4-MD2 compared to Rv0679c and other mycobacterial antigens. The Rv0679c and Rv0180c antigens have better binding energy to TLR 4-MD2 complex compared to TLR-2-1 in both human and mouse. Both antigens has better binding energy to mouse TLRs compared to human except where Rv0180c has better binding to human TLR-2-1. Our findings suggest that Rv0180c might be the preferred for binding to TLRs than Rv0679c and within TLRs, TLR-4-MD2 for interaction.

References

  1. Kaufmann, S.H, Hussey, G., Lambert, P.H. 2010. New vaccines for tuberculosis. Lancet 375: 2110–2119.
  2. Ottenhoff, T.H., 2009. Overcoming the global crisis: “yes, we can", but also for TB … ? Eur J Immunol 39: 2014–2020.
  3. Cole, S.T, Brosch, R., Parkhill J. et al. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393: 537-544.
  4. Cifuentes, D.P., Ocampo. M., Curtidor, H. 2010. Mycobacterium tuberculosis Rv0679c protein sequences involved in host-cell infection: Potential TB vaccine candidate antigen, BMC Microbiology, (4)10:109
  5. Cáceres, S.M., Ocampo, M., Arévalo-Pinzón G. et al. 2011. The Mycobacterium tuberculosis membrane protein Rv0180c: Evaluation of peptide sequences implicated in mycobacterial invasion of two human cell lines. Peptides 32:1–10.
  6. Matsuba, T., Suzuki, Y., Tanaka Y. 2007. Association of the Rv0679c protein with lipids and carbohydrates in Mycobacterium tuberculosis/ Mycobacterium bovis BCG. Archives of microbiology, 187(4):297-311.
  7. Takeda, K., and Akira S. 2001. Regulation of innate immune responses by Toll-like receptors. Jpn J Infect Dis. Dec; 54 (6):209-19.
  8. Eun-Kyeong Jo., Chul-Su Yang.,  Chul Hee Cho., and Clifford V. 2007. Harding Intracellular signalling cascades regulating innate immune responses to Mycobacteria: branching out from Toll-like receptors Cellular Microbiology, 9 (5):1087–1098.
  9. Means, T. K., Jones, B. W., Schromm, A. B. et al. 2001. Differential effects of a Toll‐like receptor antagonist on Mycobacterium tuberculosis‐induced macrophage responses. J. Immunol. 166:4074.
  10. Takeuchi, O., Sato S., Horiuchi T. et al. 2002. Cutting edge: role of Toll-like receptor 1 in mediating immune response to microbial lipoproteins. J. Immunol. 169: 10–14
  11. Shimazu, R., et al. 1999. MD-2, a molecule that confers lipopolysaccharideResponsiveness on Toll-like receptor 4. J. Exp. Med. 189, 1777–1782.
  12. Kremer, L., Maughan, W.N., Wilson, R.A. et al. 2002. The M. tuberculosis antigen 85 complex and mycolyltransferase activity. Lett Appl Microbiol. 34(4):233-7.
  13. Wilson, R.A., Maughan, W.N., Kremer, L. et al. 2004. The structure of Mycobacterium tuberculosis MPT51 (FbpC1) defines a new family of non-catalytic alpha/beta hydrolases. J Mol Biol. Jan 9;335 (2):519-30.
  14. Farrow, M.F., Rubin, E.J. 2008. Function of a mycobacterial major facilitator superfamily pump requires a membrane-associated lipoprotein. Journal of Bacteriology. 190(5):1783-1791.
  15. Supriya Shukla., Edward Richardson., Jaffre Athman. et al. 2014. Mycobacterium tuberculosis lipoprotein LprG binds lipoarabinomannan and determines its localization in the cell wall envelope and affects phagolysosomal fusion.The Journal of Immunology. vol.192 (1 Supplement) 132.18
  16. Yang, J., Yan R, Roy, A., Xu, D. et al. 2015.The I-TASSER Suite: Protein structure and function prediction. Nature Methods, 12: 7-8.
  17. Roy, A., Kucukural, A., Zhang Y. 2010. I-TASSER: a unified platform for automated protein structure and function prediction. Nature Protocols, 5: 725-738.
  18. Zhang, Y. I-TASSER server for protein 3D structure prediction. BMC Bioinformatics, vol 9, 40 (2008).
  19. Kim, H. M., Park, B. S., Kim, J. I. et al. 2007. Crystal structure of the TLR4-MD-2 complex with bound endotoxin antagonist Eritoran. Cell 130, 906–91710.1016/j.cell.2007.08.002
  20. Naito, M., Fukuda T., Sekiguchi, K., and Yamada T. 2000. The domains of human fibronectin mediating the binding of alpha antigen, the most immunopotent antigen of mycobacteria that induces protective immunity against mycobacterial infection. Biochem. J. 347, 725–731
  21. Schlesinger, L., S, and Horwitz, M. A. 1991. Phagocytosis of Mycobacterium leprae by human monocyte-derived macrophages is mediated by complement receptors CR1 (CD35), CR3 (CD11b/CD18), and CR4 (CD11c/CD18) and IFN-gamma activation inhibits complement receptor function and phagocytosis of this bacterium. J. Immunol. 147, 1983–1994
  22. Noss, E. H., Pai, R.K., Sellati, T. J., et al. 2001. Toll-like receptor 2-dependent inhibition of macrophage class II MHC expression and antigen processing by 19 kD li-poprotein of Mycobacterium tuberculosis. J. Immunol. 167: 910–918
  23. Gehring, A. J., Dobos, K. M., Belisle J. T. et al. 2004. Mycobacterium tuberculosis LprG (Rv1411c): a novel TLR-2 ligand that inhibits human macrophage class II MHC antigen processing. J. Immunol.173:2660–2668.
  24. Pecora, N.D., Gehring, A.J., Canaday, D.H. et al. 2006. Mycobacterium tuberculosis LprA is a lipoprotein agonist of TLR2 that regulates innate immunity and APC function. J Immunol. Jul 1; 177(1):422-9.

Keywords

Mycobacterium tuberculosis, toll like receptors, invasion, I-TASSER, docking, ClusPro