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Reseach Article

Screening and Characterization of Weissella cibaria isolated from food source for probiotic properties

Published on May 2014 by Elavarasi Viswanathan, Pugazhendhi Arivalagan, Poornima Priyadharshini, Valsala Haridass, Thamaraiselvi Kaliannan
National Conference cum Workshop on Bioinformatics and Computational Biology
Foundation of Computer Science USA
NCWBCB - Number 1
May 2014
Authors: Elavarasi Viswanathan, Pugazhendhi Arivalagan, Poornima Priyadharshini, Valsala Haridass, Thamaraiselvi Kaliannan
a3528df3-00e4-4a58-954c-d19ba34c358f

Elavarasi Viswanathan, Pugazhendhi Arivalagan, Poornima Priyadharshini, Valsala Haridass, Thamaraiselvi Kaliannan . Screening and Characterization of Weissella cibaria isolated from food source for probiotic properties. National Conference cum Workshop on Bioinformatics and Computational Biology. NCWBCB, 1 (May 2014), 29-32.

@article{
author = { Elavarasi Viswanathan, Pugazhendhi Arivalagan, Poornima Priyadharshini, Valsala Haridass, Thamaraiselvi Kaliannan },
title = { Screening and Characterization of Weissella cibaria isolated from food source for probiotic properties },
journal = { National Conference cum Workshop on Bioinformatics and Computational Biology },
issue_date = { May 2014 },
volume = { NCWBCB },
number = { 1 },
month = { May },
year = { 2014 },
issn = 0975-8887,
pages = { 29-32 },
numpages = 4,
url = { /proceedings/ncwbcb/number1/16510-1410/ },
publisher = {Foundation of Computer Science (FCS), NY, USA},
address = {New York, USA}
}
%0 Proceeding Article
%1 National Conference cum Workshop on Bioinformatics and Computational Biology
%A Elavarasi Viswanathan
%A Pugazhendhi Arivalagan
%A Poornima Priyadharshini
%A Valsala Haridass
%A Thamaraiselvi Kaliannan
%T Screening and Characterization of Weissella cibaria isolated from food source for probiotic properties
%J National Conference cum Workshop on Bioinformatics and Computational Biology
%@ 0975-8887
%V NCWBCB
%N 1
%P 29-32
%D 2014
%I International Journal of Computer Applications
Abstract

This study was aimed to determine the probiotic potential of lactic acid bacteria (LAB) isolated from various food sources such as cow milk, goat milk and idli batter. The strain Weissella cibaria (KTSMBNL 28) isolated from goat milk showed more antibactericidal activity than other strains from cow milk and idli batter. This strain was able to tolerate pH 3. 0 which maintained in gastric fluid and resist upto1% of bile salt retained in intestinal fluid. Moreover, KTSMBNL 28 recorded highest level of hydrophobicity (35-70%) and its non haemolytic property ensured the safe mode of application. Among the 15 antibiotics tested, KTSMBNL 28 showed resistance towards penicillin, vancomycin and nalidixic acid. Therefore, Weissella cibaria KTSMBNL 28 which showed good probiotic properties and could be attribute beneficial effects to mankind.

References
  1. Chou, L. and Weimer, B. 1999, Isolation and characterization of acid and bile- tolerant isolates from strains of Lactobacillus acidophilus. J. Dairy Sci. 82, 23-31.
  2. Stiles, M. E. and Holzapfel, W. H. 1997, Lactic acid bacteria of foods and their current taxonomy, Int. J. Food Microbiol. 36, 1–29.
  3. Björkroth, K. J. , Schillinger, U. , Geisen, R. , Weiss, N. , Hoste, B. , Holzapfel, W. H. , Korkeala, H. J. and Vandamme, P. 2002, Taxonomic study of Weissella confusa and description of Weissella cibaria sp. nov. , detected in food and clinical samples, Int. J. Syst. Evol. Microbiol. 52, 141-8.
  4. De Vuyst, L. , Schrijvers, V. , Paramithiotis, S. , Hoste, B. , Vancanneyt, M. , Swings, J. , Kalantzopoulos, G. , Tsakalidou, E. and Messens, W. 2002,The biodiversity of lactic acid bacteria in Greek traditional wheat sourdoughs is reflected in both composition and metabolite formation, Appl. Environ. Microbiol. 68, 6059-69.
  5. Santos, E. M. , Jaime, I. , Rovira, J. , Lyhs, U. , Korkeala, H. and Björkroth, J. 2005, Characterization and identification of lactic acid bacteria in "morcilla de Burgos", Int. J. Food Microbiol. 97, 285-96.
  6. Klaenhammer, T. R. 1993, Genetics of bacteriocins produced by lactic acid bacteria, FEMS microbial. reviews 12, 39-85.
  7. Cleveland, J. , Montville, T. J. , Nes, I. F. and Chikindas, M. L. 2001, Bacteriocins: Safe, natural antimicrobial for food preservation, Int. J. Food Microbiol. 71, 1-20.
  8. Ogunbanwo, S. T. , Sanni, A. I. and Onilude, A. A. 2003, Characterization of bacteriocin produced by Lactobacillus plantarum F1 and Lactobacillus brevis OG1, Afr. J. Biotechnol. 2, 219-227.
  9. Vijai-Pal, J. M. and Jeevarathnam, K. 2005, Isolation and characterization of bacteriocin producing lactic acid bacteria from a south Indian special dosa (Appam) batter, J. Culture Collection, 4, 53-60.
  10. Schillinger, U. and Lücke, F. K. 1989, Antibacterial activity of Lactobacillus sake isolated from meat, Appl. Environ. Microbiol. 55, 1901-6.
  11. Mourad, K. and Eddie, K. N. 2006, In vitro preselection criteria for probiotic Lactobacillus plantarum strains of fermented olives origin, Int. J. Probiotics and Prebiotics, 1,27-32.
  12. Prasad, J. , Gill, H. , Smart, J. and Gopal, P. K. 1998, Selection and Characterization of Lactobacillus and Bifidobacterium strains for use as probiotic,Int. Dairy J. 8, 993-1002.
  13. Erkkilä, S. and Petäjä, E. 2000, Screening of commercial meat starter cultures at low pH and in the presence of bile salts for potential probiotic use, Meat Science, 55 , 297–300.
  14. Kiely, L. J. and Olson, N. F. 2000, The physiochemical surface characteristics of Lactobacillus casei, Food Microbiol. 17, 277-291.
  15. Rojas, M. , Ascencio, F. and Conway, P. L. 2002, Purification and characterization of a surface protein from Lactobacillus fermentum 104R that binds to porcine small intestinal mucus and gastric mucin, Appl. Environ. Microbiol. 68,2330-2336.
  16. Ross, S. and Jonsson, H. 2002, A high – molecular mass cell surface protein from Lactobacillus reuteri 1063 adheres to mucus components, Microbiology, 148, 433-442.
  17. Mathur, S. and Singh, R. 2005, Antibiotic resistance in food lactic acid bacteria - a review, Int. J. Food Microbiol. 105, 281-295.
  18. Ammor, M. S. , Florez, A. B. and Mayo, B. 2007, Antibiotic reistance in non-enterococcal lactic acid bacteria and bifidobacteria, Food Microbiol, 24, 559-570.
Index Terms

Computer Science
Information Sciences

Keywords

Weissella Cibaria Antibactericial Activity Acid And Bile Tolerance Antibiotics Resistance.