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Mathematical Modeling of Mist bioreactor for the Growth of Hairy Roots
| National Workshop-Cum-Conference on Recent Trends in Mathematics and Computing 2011 |
| Foundation of Computer Science USA |
| RTMC - Number 13 |
| May 2012 |
| Authors: K. Sambasiva Rao, Rajesh Khanna, Ritu Ranjan |
| 41dd5378-163d-46d5-abf5-aedbc79658a6 |
K. Sambasiva Rao, Rajesh Khanna, Ritu Ranjan . Mathematical Modeling of Mist bioreactor for the Growth of Hairy Roots. National Workshop-Cum-Conference on Recent Trends in Mathematics and Computing 2011. RTMC, 13 (May 2012), 16-20.
Abstract
Plant derived chemicals are valuable sources for a variety of pharmaceuticals, flavors, dyes, fragrance, stimulants, poisons. Hairy roots are induced in susceptible plants by transformation with Agrobacterium rhizogenes. Here we summarize the recent work using a novel bioreactor, the mist reactor, for the culture of hairy roots from many plant species. This study is based on the development of a mathematical model at root bed level per unit mass basis. Model equations were developed to study the operating parameters for constant specific growth rate (?) processes. Important parameters for the NMR operation, effective mist flow rate, nutrient concentration available to roots, duration of mist-ON/mist-OFF cycle, capture efficiency and drainage characteristics of the root bed. A new method to describe the drainage characteristics of the hairy root bed is shown in this study. It is clear from the results, the mist ON/OFF cycles play an important role in an efficient operation of mist reactor and good growth rate can be achieved either by increasing nutrient concentration or by changing the mist duty cycles
References
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